Dynamics of cAMP formation in live primary hippocampal neurons (DIV 4) using the cAMP FRET sensor, EPAC1. cAMP FRET was performed on ROI1 and ROI2 (cell body). The images show the calculated nF prior to addition of isoproterenol (panel A) and 5 min post addition (10mM, panel B) This experimental cAMP FRET was then compared with the simulation results (panel C) in the corresponding locations.

Solutions for FRET

UltraVIEW VoX for FRET

UltraVIEW VoX is our complete spinning disk confocal system designed to meet the challenges of your live cell imaging. A wide range of excitation wavelengths means that you have a choice of fluorophore pairs.

Volocity FRET analysis

Volocity is our advanced 3D–4D imaging software which includes a range of easy-to-use tools for complete analysis of your FRET data.   FRET analysis allows accurate FRET measurements and generation of net FRET (or "corrected" FRET) and normalized FRET images for publication.