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Dr. Vipoir's Tip of the Month

A collection of articles describing ways of getting the most out of  cellular imaging products.

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A forum for discussing products and services for PerkinElmer's cellular imaging & analysis products.

Technical Notes

Title Product Modification Date Views
System Requirements for Volocity Product Range Volocity 18/12/2009 10035
Volocity Supported Hardware Volocity 18/12/2009 12568
Limitations of Hamamatsu ImagEM "Real-time Image Processing" Features Other 07/12/2009 100
UltraVIEW VoX - Optimizing Acquisition Frame Rate Volocity 09/07/2009 367
Using the Zeiss AxioObserver Definite Focus with Volocity Acquisition Volocity 07/07/2009 375
Using the Nikon TiE PFS with Volocity Acquisition Volocity 06/07/2009 470
Volocity for Windows x64 Volocity 15/04/2009 2690
Openlab Supported Hardware Openlab 27/03/2009 11687
Volocity Surface Area Measurements Volocity 14/01/2009 222
Volocity and Windows Vista Volocity 17/11/2008 1927
System Requirements for Volocity LE Volocity 02/10/2008 1293
ILS System Requirements ILS 13/08/2008 2483
Using the Olympus IX81 ZDC with Volocity Acquisition Volocity 25/07/2008 1115
Additional TIFF Information in Phylum and Volocity Phylum and Volocity 11/06/2008 2800
System Specifications for Imaging Computing Server Volocity 02/04/2008 1953
Openlab 5.5 Openlab 27/03/2008 1135
Volocity for Windows: Optimizing performance Volocity 28/02/2008 3463
Using the Nikon PFS with Volocity Acquisition Volocity 13/08/2007 1284
Spectral Separation of Monochrome Images Using Volocity 4 Volocity 13/08/2007 1143
Volocity and Neurolucida Volocity 30/03/2007 1299
Openlab 5: Color Tables and Automator Items Openlab 21/12/2006 1361
Finding Dirt and Debris in your Microscope Other 24/11/2006 1687
Diagnosing Microscope Drift Problems Openlab 22/11/2006 1466
Improvision Micro-Shutter performance Phylum Live 02/05/2006 400
Openlab 5: New Location for Color Tables, Automator Menu Items and Automator Subroutines Openlab 28/04/2006 1791
Openlab and Mac OS X Openlab 16/01/2006 8488
Improvision Micro-Shutter performance Other 04/01/2006 5411
A study of fluorescence standards confirms that OptiGrid confocal images are suitable for quantitative microscopy Phylum Live 22/08/2005 689
How do I transfer files between a PC and a Mac? Openlab 19/08/2005 9917
How does Openlab work with different image formats? Openlab 19/08/2005 1981
Which image files are compatible with Openlab? Openlab 19/08/2005 2865
How can I open TIFF Images with Openlab? Openlab 19/08/2005 1973
Why does there appear to be degradation to the resolution of the graphics in an annotation layer compared to that of a measurement overlay? Openlab 19/08/2005 2358
How can I create my own columns in the Advanced Measurements table? Openlab 19/08/2005 2009
What is a typical practical value for the accuracy and repeatability of the PIFOC piezo focus drive? Openlab 19/08/2005 2402
How do I export images from Openlab as individual TIFFs for use in other graphics applications? Openlab 19/08/2005 2318
How do I optimise memory in Mac OS 9 for use with Openlab? Openlab 19/08/2005 1871
How do I save the color LUT when exporting images into Adobe Photoshop? Openlab 19/08/2005 2180
Why is the device connected to the Serial Port of my computer not communicating with Openlab? Openlab 19/08/2005 2070
Why am I only able to capture images at a reduced size rather than full image size? Openlab 19/08/2005 1557
Controlling the VMM-D3 Three Channel Shutter Driver using Openlab Automator Serial Communications Openlab 19/08/2005 2154
Volocity for Windows - Dongle Installation Volocity 19/08/2005 2062
Using Openlab Layer User Data to set properties within Volocity Volocity 19/08/2005 1806
How do I run serial devices from a Mac which has only USB ports? Openlab 26/07/2005 1750
Year 2000 Statement Openlab 26/07/2005 1722
Achieving accurate color reproduction for brightfield microscopy using a color camer. Openlab 25/07/2005 1845
How do I make a scale bar to stamp on my images in Openlab? Openlab 25/07/2005 3262
Openlab Raw Format Openlab 09/11/2004 3763
Openlab Module - User Data Openlab 11/03/2004 1756
Ludl Controller Configuration Openlab 11/03/2004 2080
How do I density slice an image? Openlab 12/02/2004 3063
How do I edit the settings on my Openlab Filterwheel palette? Openlab 12/02/2004 1750
How do I set up the Dage DC330 color camera for transmitted light microscopy in Openlab? Openlab 12/02/2004 1904
Openlab User Interface Concepts Openlab 12/02/2004 1673
Can Phylum archive movie files? Phylum 12/02/2004 1984
How do I calibrate my TILL Photonics Polychrome monochromator in Openlab? Openlab 12/02/2004 2065
How do I use the CRI Micro Color filter with Openlab? Openlab 12/02/2004 2005
How do I prevent erratic timings when capturing large sequences of images in Openlab? Openlab 12/02/2004 1682
Does the Oprnlab Automator support array variables? Openlab 12/02/2004 1828
How do I superimpose results from the Measurements table onto an image layer? Openlab 12/02/2004 2072
How can I open images acquired with older Improvision imaging products in Openlab? Openlab 12/02/2004 1911
Power Macintosh G3 and G4 computers using Mac OS 9 with the National Instruments PCI 1200 card. Openlab 12/02/2004 1834
How do I measure the optical density of objects using Openlab? Openlab 12/02/2004 3220
How do I set up and use the the multi-user facility in Openlab? Openlab 12/02/2004 1353
How can I record a variable in an Openlab automation to be exported to a data analysis program such as Excel? Openlab 12/02/2004 1648
Where can I find the Openlab serial number? Openlab 12/02/2004 1845
Why do Openlab images look poor on the monitor? Openlab 12/02/2004 1498
Why does my automation refuse to stop during a Tell or Input task? Openlab 12/02/2004 1525
Why does my automation refuse to pause during a Tell task? Openlab 12/02/2004 1474
How can I determine the exact degree of image registration that is needed to re-align my multilabelled fluorescent image Openlab 12/02/2004 1608
Why can't I import Adobe Photoshop files into Phylum? Phylum 12/02/2004 1775
How do I move images from different Openlab documents into one Openlab document? Openlab 12/02/2004 1565
Keyboard Shortcuts for use with Openlab. Openlab 12/02/2004 1668
How do I convert the Openlab layer time stamp from days:hours:mins:sec into seconds so that I can use the data in Excel? Openlab 12/02/2004 1788
Why can't I annotate my images in white text using a transparent annotation layer in Openlab? Openlab 12/02/2004 1725
How do I track the speed and position of cells in a time lapse sequence with Openlab? Openlab 12/02/2004 1929
How do I make a QuickTime movie in Openlab? Openlab 12/02/2004 1731
How can I make an automation go faster? Openlab 12/02/2004 1586
Why can't I annotate my images using the Paint Tools palette? Openlab 12/02/2004 1646
Why does the image sequence that I saved as a QuickTime movie look pixelated? Openlab 12/02/2004 1840
How can I export graphs generated in Openlab into other applications ? Openlab 12/02/2004 1607
How do I reset the Point Counter so that I can begin a new count on each layer? Openlab 12/02/2004 1500
I am using OpenLab with Mac OS 9.2.2 and I am repeatedly getting out of memory errors when I try different commands. What should I do? Openlab 12/02/2004 1542
I am using binning to increase the sensitivity of my camera. I can view the image in preview mode but get an error message when I try to capture the image. Openlab 12/02/2004 1546
I’m selecting an ROI measurement from the Measurements Table, but Openlab is not displaying either the ROI or the image that it corresponds to in the document window. Openlab 12/02/2004 1699
I have downloaded the latest version of Openlab from www.improvision.com. Now what do I do? Openlab 12/02/2004 1553
How do I change the color of the paint tools? Openlab 12/02/2004 1578
What should I know about the 3D Restoration module? Openlab 12/02/2004 1715
How can I focus the SPOT RT camera quickly? Openlab 12/02/2004 1741
How can I play a movie in Phylum which doesn't include all of the images in the folder? Phylum 12/02/2004 1633
Why doesn't the software recognize my camera after I have installed a new version of Openlab? Openlab 12/02/2004 1800
Why can’t I apply a color look up table (LUT) to my merged images? Openlab 12/02/2004 1810
Which way should filters go into my filter wheel? Other 12/02/2004 1717
I can't see the line that has been drawn when I create an ROI or use the measurement tools in Openlab. Can I change the color? Openlab 12/02/2004 1682
When I use the RGB merge to merge a slightly opaque layer with other layers, why is the resultant image of poor quality, and why is detail in the opaque layer lost? Openlab 12/02/2004 1912
Why is only the Layers Manager palette available when I start up Openlab? Openlab 12/02/2004 1635
Can I use 8 or 12 bit TIFF files generated by other systems within Openlab? Openlab 12/02/2004 1777
Why am I getting a grainy black and white image on my color CoolSNAP camera? Openlab 12/02/2004 1935
My camera has non symmetrical pixels. Which value do I enter for the pixel size in the deconvolution dialog box? Openlab 12/02/2004 1569
How do I use the AxioCam camera with Openlab? Openlab 12/02/2004 1833
Zeiss Axioplan 2ie and Axiovert 200M behave strangely with Openlab 2 Openlab 12/02/2004 2470
Openlab 3 - The Filters and Shutters module now supports 'link to video' for shutters. Openlab 12/02/2004 1616
Increased functionality for the Automator Lite in Openlab 3 Openlab 12/02/2004 1498
What features of the Hamamatsu 4742-95ER (Orca ER) are now supported in Openlab 2.2.5r2? Openlab 12/02/2004 2161
Auto Exposure in Openlab Openlab 12/02/2004 1819
Preferences Issues When Moving Between Openlab versions 2 and 3. Openlab 12/02/2004 1510
Openlab 3 - Auto Focus now supported for motorised focus drives Openlab 12/02/2004 1652
Using Roper (Princeton Instruments) cameras with the PVCAM Video module Openlab 12/02/2004 2696
Can I make a binary mask using the Binary Operations module? Openlab 12/02/2004 2252
Why is the integration of my Hamamatsu C5810 camera not synchronised within an automation? Openlab 12/02/2004 2025
How do I save spatial calibrations for different objectives in Openlab? Openlab 12/02/2004 1594
Which cameras will speed up significantly when using the Automator "Capture Multiple" task? Openlab 12/02/2004 1879
What is the UV throughput of the fiber optic supplied with the TILL monochromators and are they solid quartz? Openlab 12/02/2004 1926
How do I use Openlab to measure the greyscale intensity values for a number of different ROIs over the course of a timelapse experiment (i.e. over several layers)? Openlab 12/02/2004 1712
I lost the power to the Openlab acquisition system and lost the image file I had captured. Can I get it back? Openlab 12/02/2004 1651
Can I use more than one Ludl controller with Openlab? Openlab 12/02/2004 1631
How do I remove the number from my cell tracking overlay in Openlab? Openlab 12/02/2004 1529
I cannot get communication with my ORBIT 1 controller in Openlab. Openlab 12/02/2004 1640
How do you make a 3D profile in Openlab? Openlab 12/02/2004 1648
How can I measure grayscale intensity values over an image using the same ROI? Openlab 12/02/2004 1892
When I draw an ROI on an image, it won't retain the shape I have drawn. Openlab 12/02/2004 1568
How do I change size of image in order to speed up capture and/or processing time? Openlab 12/02/2004 1540
Changes in Openlab 3 to the Automator Controls palette Openlab 12/02/2004 1648
"Save as Multiple" and file naming in Openlab Openlab 12/02/2004 1789
How do I measure the total intensity of a cell or ROI in Openlab? Openlab 12/02/2004 1865
What version of Mac OS can I use with Openlab? Openlab 12/02/2004 1826
Openlab 3.0.2 - Target Image Window Automator task - new option Openlab 12/02/2004 1806
Orbit Focus Drives - Preferences Openlab 12/02/2004 1824
Volocity 3D Rendering - how to export a 2D view Volocity 12/02/2004 1668
Write to User Notes Automator task - Tab function Openlab 12/02/2004 1813
Open Image Document Automator Task Openlab 12/02/2004 1600
Save Document Automator Task - format options Openlab 12/02/2004 1691
When using the "Volume Deconvolution" module in Openlab, the final deconvolved image does not resemble the image in the preview window. Openlab 12/02/2004 1473
Using Volocity, I have enhanced my images by using the Auto Contrast function but my volume does not look as expected. Volocity 12/02/2004 1537
Openlab Microscope Control - Light Path Automator task Openlab 12/02/2004 1598
Set Video Control Automator task - changing check boxes and pop up choices Openlab 12/02/2004 1556
Ludl Biopoint controllers do not fully support State Saver operations. Openlab 12/02/2004 1787
Alternate Readout Mode for Photometrics PVCAM cameras, specifically the CoolSNAP HQ. Openlab 12/02/2004 2130
Why is the progress bar on the Video Controls palette always moving? It never becomes a solid block, is this correct? Openlab 12/02/2004 1551
Zeiss AxioCam color camera - White Balance for brightfield microscopy. Openlab 12/02/2004 2247
Roper Scientific Photometrics color CoolSNAP camera - White Balance for brightfield microscopy. Openlab 12/02/2004 1962
How do I connect serial devices to Macs which do not have serial ports? Openlab 12/02/2004 6884
Openlab Module - 3D Rendering - using the 'Scale with Z' option Openlab 12/02/2004 1696
Volocity - Alignment tool Volocity 12/02/2004 1684
Leica DMSTC Motorised XY Stage and CTRMIC microscopes in Openlab Openlab 12/02/2004 2452
ORBIT 1 XY Stage Openlab 12/02/2004 1841
An example automation illustrating the use of multithreading Openlab 12/02/2004 1680
An example automation illustrating the use of parallel processing and the "Key wait" task Openlab 12/02/2004 1609
How to create an automation to measure objects and put the results into a graph. Openlab 12/02/2004 1599
Openlab Module - Colocalization Openlab 12/02/2004 1759
Openlab Module - Image Splitter Openlab 12/02/2004 1755
Spatial calibration in Openlab 3.1 and higher Openlab 12/02/2004 1745
How to install Openlab Darkroom Openlab 12/02/2004 1559
QImaging Camera Firmware Upgrade Openlab 12/02/2004 1818
Openlab Ratio Module: Intensity Modulated image Openlab 12/02/2004 1726
Openlab Module - FRET Openlab 12/02/2004 1668
Elongation of Spherical Structures in Z Other 12/02/2004 1689
Volocity LE 2.5 and higher Volocity 12/02/2004 1582
Openlab Automator Serial Communications Openlab 12/02/2004 1720
         
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