Opera

The Opera™ is the premier confocal microplate imaging reader that provides high throughput and high content screening, and generates high quality results fast.

Combining superior resolution and speed of operation with our flexible image analysis software Acapella™, the system serves an increasing demand in drug discovery and high throughput biology.

For an automated solution the Opera integrates seamlessly with our robotic workstations for live cell or fixed cell assays. They can be extended to our cell::explorer™, a fully integrated, ready to run automation system customized to meet your needs.

Key Features

• Ultra-high speed multi color parallel data acquisition
• High speed online image analysis powered by parallel computing and multi-core processors
• Exceptional image quality
• Fully automated high NA water immersion objectives for the highest performance
• Prevents photobleaching and phototoxicity for live cell assays
• High throughput enabling primary and secondary screening campaign

Options

• Non-confocal UV - additional channel for optimal excitation of UV nuclear dyes.
• Environmental control of temperature, CO2 and humidity - optimal conditions for live-cell applications.
• On-board dispenser, independent movement of dispenser head and scanning table and software for nested dispense-and-read protocols - unique opportunity for high throughput kinetic applications.
• Fluorescence Lifetime Imaging (FLIM) version - High Content Screening using probes with fluorescence lifetime in the nanosecond range for molecular interaction and FRET approaches.

Opera QEHS

The Opera™ QEHS provides four color parallel image acquisition. Four lasers plus UV lamp, up to four CCD cameras and 14 fluorescence emission options serve for maximum flexibility at the highest speed.

Opera LX

The Opera™ LX is a starter configuration of the Opera for three color applications that can be extended as your High Content Analysis needs grow.

Opera FLIM

The Opera™ FLIM is a special version of the Opera QEHS for time-domain fluorescence lifetime imaging based screening. The 405 nm laser is replaced by a pulsed laser and one of the confocal cameras is an intensified gated ICCD camera. Fluorescence lifetime is measured for every camera pixel leading to an intensity independent and robust readout, for example protein-protein interactions using FRET.

For each version of Opera, you can choose from a number of upgrades such as environmental control for live cell applications, multi-color parallel image acquisition and automation options.

Assay Groups:

Whichever assay you are working on, the Opera HCS reader offers a solution:

• Cytotoxicity
• Apoptosis
• Calcium Flux
• Cell cycle
• Cell proliferation
• Cell differentiation
• Cell shape changes
• Receptor activation
• Transcription factors
• Cytoskeletal rearrangement
• Protein expression
• Signaling pathway analysis
• RNAi screening
• Neurite outgrowth
• Lipid droplet analysis
• Fluorescence in situ hybridization (FISH)