Driven by your biology - instruments,
software and expert support
for cellular imaging and analysis
Harmony Ready-Made Solutions
The Harmony(™) software includes a full range of ready-made solutions for common image analysis tasks and assays that are pre-installed for your convenience.
| Proliferation | |
Proliferation |
Cell counting or Nuclei counting |
| Cell Cycle | |
Transition of G2 to M-phase (Cyclin B1) |
Quantification of marker in cytoplasm (modified to capture the whole cell fluorescence including the nucleus) |
Mitotic index (phosphohistone H3) |
Mitotic index |
S-Phase (BrdU, EdU) |
Quantification of Marker in Nucleus |
DNA content |
Nuclear classification - DNA content |
Simultaneous phosphohistone H3, EdU, DNA content and nuclear morphology |
Cell Cycle Classification |
| Cytotoxicity | |
Cell count |
Cell counting or Nuclei counting |
Cell viability: live/dead cell count |
Live / dead cell counting |
Nuclear swelling and shrinking |
Nuclear analysis - nuclear shrinking |
Loss of mitochondrial membrane potential |
Quantification of marker in cytoplasm |
Mitochondrial mass |
Quantification of marker in cytoplasm |
Calcium homeostasis |
Quantification of marker in cytoplasm |
Membrane integrity |
Live / dead cell counting |
Phospholipidosis |
Quantification of marker in cytoplasm |
Hepatosteatosis |
Quantification of marker in cytoplasm |
Peroxisome quantification |
Spot analysis or Quantification of marker in cytoplasm |
| Apoptosis | |
Nuclear fragmentation |
Nuclear fragmentation |
Activation of Caspase-3 |
Quantification of marker in nucleus (using enlarged nucleus region to capture fluorescence in cytoplasm area next to the nucleus as well) |
Mitochondrial mass |
Quantification of marker in cytoplasm |
Loss of mitochondrial membrane potential |
Quantification of marker in cytoplasm |
| Protein expression | |
In nucleus |
Quantification of marker in nucleus |
In cytosol |
Quantification of marker in cytoplasm |
In plasma membrane |
Quantification of marker in membrane |
| Receptor activation | |
GPCR activation: Endocytosis |
Receptor internalization |
GPCR activation: arrestin recruitment (e. g. Transfluor®) |
Spot analysis |
Labelled ligand internalization |
Receptor internalization |
pH-sensitive Dye Mediated Internalization |
Receptor internalization |
| Signalling pathways | |
Transcription factor activation |
Cytosol to nucleus translocation |
Cytoplasm to nucleus translocation |
Cytosol to nucleus translocation |
Nucleus to cytoplasm translocation |
Cytosol to nucleus translocation |
Cytoplasm to membrane translocation |
Cytosol to membrane translocation |
Membrane to cytoplasm translocation |
Cytosol to membrane translocation |
Membrane ruffling |
Quantification of marker in membrane |
| Vesicle Formation | |
Vesicle formation |
Spot analysis |
| FISH | |
FISH |
Nuclear spots |
| Cytoskeletal rearrangement | |
Cytoskeletal rearrangement |
Fluorescence redistribution – cytoskeleton |
| Cell spreading | |
Cell spreading |
Cell shape – cell rounding |
| Cell detachment | |
Cell detachment, cell rounding |
Cell shape – cell rounding |
| Neurite Outgrowth | |
Neurite outgrowth |
Neurite analysis |