Acapella Script Collection

It’s imperative that image analysis software for High Content Screening (HCS) applications includes a large toolbox of image analysis modules and a wide range of ready-made applications which are easy to understand and apply. The ability to adjust imaging algorithms to applications and cell types as well as combine algorithms for multiplexed readout is also crucial. The powerful and intuitive Acapella addresses these requirements and provides tools for every user, from beginners to experts.

The software includes the Acapella Script Collection, a large library of ready-made image analysis solutions for frequently used HCS assays such as translocation, receptor activation / endocytosis, signal molecule recruitment, cell cycle, cell viability, and many more.

The collection of ready made scripts also includes a short description of the corresponding assay and demo images as well.

Whole Cell Fluorescence

• Cell Counting
• Live/Dead Assay
• Stem Cell Differentiation Assay Using Cytosol Marker
• Calcium Flux Kinetics Assay

Sub Cellular Compartment Fluorescence

• Nuclei Counting
• General Nuclear Characterization
• General Cell Characterization
• General Multi Parameter Spot Analysis
• Mitotic Index Assay
• Nuclear Spot Detection Assay

Fluorescence Translocation

• AKT 3 Cytoplasm to Cell Membrane Translocation Assay
• FKHR Cytoplasm to Nucleus Translocation Assay Example
• GPCR Receptor Internalization Assay Example
• Transfluor GPCR Receptor Internalization
• NFkB Cytoplasm to Nuclei Translocation Assay Example

Morphology

• Cell Cluster Analysis
• Neurite Outgrowth Assay Example
• Nuclear Fragmentation - Not released in AC 2.0 yet -
• Cell Rounding
• Mitotic Classification