Neurite Outgrowth

Regeneration of neurons represents a promising strategy for drugs targeted against neurodegenerative injuries and disorders such as Alzheimer’s and Parkinson’s disease. The development of new therapies is focused on identifying molecules that affect the differentiation of neurons and neurite outgrowth.

For this purpose automated measurement and analysis of neuronal cells is essential for neuroscience research and drug discovery. Neurotoxicity is another important area where neurite outgrowth analysis is applied.

Impact of NGF on neurite outgrowth of NS-1 cells. Confocal images of control (left panel) and NGF treated (right panel) NS-1 cells. Hoechst 33342 stained nuclei are shown in blue. α-tubulin was labeled by immunofluorescence with Alexa Fluor® 488 and is shown in green.

Our Neurite Outgrowth solutions

Drawing on many years of experience and in-depth knowledge, PerkinElmer offers solutions for every throughput, a starting point for typical neurite outgrowth assays and unlimited possibilities for customization:

Opera High Content Screening System

• High sample throughput with multi-color parallel image acquisition and over 100,000 data points per day

Operetta Compact High Content Screening System

• High speed with large field of view and large cell number captured with every image
• Ready-made image analysis sequences based on CSIRO algorithms creating number of cells, total length of neuritis, longest neurite of a cell, number of root neuritis, number of branch points, number of extreme neurites and documentation on how to customize

Acapella Image Analysis Software

• Image analysis sequences based on CSIRO algorithms creating number of cells, total length of neuritis, longest neurite of a cell, number of root neuritis, number of branch points, number of extreme neurites
• Unlimited possibilities for adding parameters and modifying readout

Columbus High Volume Data Management and Analysis System

• Enterprise level data management from assay development to high throughput screening seamlessly linking high content data, research data, in vivo imaging data, result management, screening statistics, biological and chemical data

cell::explorer Automated High Content Screening System

• Full environmental control and workstation with hand-over of plates from imager to incubator for short-term and long-term live cell assays

You may also be interested in: Live Cell Imaging.