Cytoskeletal Rearrangement

The cytoskeleton, a filamentous network of actin, tubulin and other protein components, is essential for cellular structure, maintenance, intracellular transport, cell division and many other functions.

Cytoskeletal rerarrangements occur in physiological events such as cell movement. Cytoskeletal defects are frequently associated with diseases such as cancer / metastasis and also cytotoxicity. Monitoring the cytoskeletal structure is a very sensitive High Content Analysis in many application areas.

Untreated cells – structured distribution of tubulin with high intensity near the nucleus and decreasing intensity near the plasma membrane. Re-arrangement of tubulin due to treatment with Demecolcin.

Our Cytoskeletal Rearrangement solutions

Drawing on many years of experience and in-depth knowledge, PerkinElmer offers solutions for every throughput, a starting point for typical cytoskeletal rearrangement assays and unlimited possibilities for customization:

Opera High Content Screening System

• High sample throughput with multi-color parallel image acquisition and over 100,000 data points per day

Operetta Compact High Content Screening System

• High speed with large field of view and large cell number captured with every image
• Ready-made image analysis sequences for cytoskeletal distribution and texture based analysis including documentation on how to customize
• Unlimited possibilities for adding parameters and modifying readout

Acapella Image Analysis Software

• Image analysis tools of maximum flexibility for evaluation based on cytoskeletal staining, cytoskeletal fibre identification, cytoskeletal texture and cell shape
• Unlimited possibilities for adding parameters and modifying readout

Columbus High Volume Data Management and Analysis System

• Enterprise level data management from assay development to high throughput screening seamlessly linking high content data, research data, in vivo imaging data, result management, screening statistics, biological and chemical data

cell::explorer Automated High Content Screening System

• Full environmental control and workstation with hand-over of plates from imager to incubator for short-term and long-term live cell assays

You may also be interested in: Live Cell Imaging.