Cell Migration

Analysis of cell migration is an important phenotypical assay of processes such as tumor metastasis, immune response and wound healing.

There are four  different types  of cell migration: migration of cells based on a chemical environment (chemotaxis); cell migration within a gradient of chemoattractants (haptotaxis); movement of cells through the vascular endothelium (transmigration), and migration of cells into a wound to close the gap (wound healing).

Most of these assays are compatible with an imaging based readout. Recent strategies have resulted in more advanced cell migration assays that are more accurate, sensitive, convenient and robust, especially in wound healing. 

Oris™ Cell Migration Assay Adherent cell migration assay without the need for transmembrane inserts. Alternative to scratch assay for wound healing studies, Oris™ 96-well Collagen I Coated plates with Oris™ Cell Seeding Stoppers; HT-1080 cell line before (left) and 18 hr after (right) migration into the ‘wound’ area.

Our Cell Migration solutions

Drawing on many years of experience and in-depth knowledge, PerkinElmer offers solutions for every throughput, a starting point for typical cell migration assays and unlimited possibilities for customization:

Opera High Content Screening System

• High sample throughput with multi-color parallel image acquisition

Operetta Compact High Content Screening System

• High speed with large field of view and large cell number captured with every image
• Image analysis building blocks for evaluation based on area, cell number, cytoskeletal staining and cell shape

Acapella Image Analysis Software

• Image analysis tools of maximum flexibility for evaluation based on area, cell number, cytoskeletal staining and cell shape
• Unlimited possibilities for adding parameters and modifying readout

Columbus High Volume Data Management and Analysis System

• Enterprise level data management from assay development to high throughput screening seamlessly linking high content data, research data, in vivo imaging data, result management, screening statistics, biological and chemical data

cell::explorer Automated High Content Screening System

• Full environmental control and workstation with hand-over of plates from imager to incubator for long-term live cell assays

You may also be interested in: Live Cell Imaging.