Cell Differentiation

Cell differentiation is a multi-step procedure associated with the expression of a specific set of cellular markers in each step, for example the progression of a pluripotent stem cell to a progenitor cell and a fully differentiated tissue specific cell type. The morphology of the cell also changes during differentiation.

Classification of GFAP positive (green squares), Tuj1 positive (red squares) and GFAP/Tuj1 positive cells (green and red squares) in a mixed population of neuronal and Glia cells.

Our Cell Differentiation solutions

Drawing on many years of experience and in-depth knowledge, PerkinElmer offers solutions for every throughput, a starting point for typical cell differentiation assays and unlimited possibilities for customization:

Opera High Content Screening System

• High sample throughput with multi-color parallel image acquisition and over 100,000 data points per day

Operetta Compact High Content Screening System

• High speed with large field of view and large cell number captured with every image
• Ready-made image analysis sequences for quantification of markers in nucleus, cytosol and membrane, neurite outgrowth and documentation on how to customize
• Building blocks for morphometric parameter assessment, such as texture, area, roundness or width/length ratio

Acapella Image Analysis Software

• Image analysis sequences for quantification of markers in nucleus, cytosol and membrane, neurite outgrowth
• Tools for morphometric parameter assessment, such as texture, area, roundness or width/length ratio
• Unlimited possibilities for adding parameters and modifying readout

Columbus High Volume Data Management and Analysis System

• Enterprise level data management from assay development to high throughput screening seamlessly linking high content data, research data, in vivo imaging data, result management, screening statistics, biological and chemical data

cell::explorer Automated High Content Screening System

• Full environmental control and workstation with hand-over of plates from imager to incubator for short-term and long-term live cell assays

You may also be interested in: Live Cell Imaging.