Infection

Traditionally an area of flow cytometry, many infection assays have been transferred from flow cytometry to imaging. High content analysis of cellular infection with pathogens encompasses a large number of different processes to which an imaging based readout can provide essential insights.

It starts with monitoring interactions between a pathogen and the cell surface and the entry of the pathogen into the cell. It involves the understanding of cellular signal transduction and transcriptional response to pathogen infection, all the way to real-time monitoring of cell viability during an infection process.

High resolution confocal imaging, as provided by our Opera™ and Operetta™ instruments, is an essential technology for a number of assays for infectious diseases. It enables 'mix and measure' homogeneous assays without the need for 'wash away' steps greatly simplifying the assay set-up process for high throughput screening, e.g. when adding an excess of labeled antibody. High resolution imaging is essential for distinguishing punctuate signals from evenly distributed signals, for example when analyzing association of viral spikes with lipid rafts.

Our Infection Assay Solutions

Drawing on many years of experience and in-depth knowledge, PerkinElmer offers solutions for every throughput, a starting point for typical infection assays and unlimited possibilities for customization:

Opera High Content Screening System

• High sample throughput with multi-color parallel image acquisition and over 100,000 data points per day

Operetta Compact High Content Screening System

• High speed with large field of view and large cell number captured with every image
• Ready-made image analysis sequences for e. g. viral entry based on spot analysis and documentation on how to customize
• Unlimited possibilities for adding parameters and modifying readout

Acapella Image Analysis Software

• Image analysis sequences for e. g. viral entry based on spot analysis
• Unlimited possibilities for adding parameters and modifying readout

Columbus High Volume Data Management and Analysis System

• Enterprise level data management from assay development to high throughput screening seamlessly linking high content data, research data, in vivo imaging data, result management, screening statistics, biological and chemical data

cell::explorer Automated High Content Screening System

• Full environmental control and workstation with hand-over of plates from imager to incubator for short-term and long-term live cell assays

You may also be interested in: Live Cell Imaging.