Driven by your biology - instruments,
software and expert support
for cellular imaging and analysis
Opera Application Notes
Analysis of the Forkhead Redistribution® Assay using the Opera
Transcription factors such as FKHR function as key regulators in e.g. insulin signalling, cell cycle progression and apoptosis downstream of phosphoinositide 3-kinase (PI3K). Inactive FKHR is cytoplasmic but is rapidly imported to the nucleus upon inactivation of the PI3K/Akt pathway. The Forkhead Redistribution® Assay is designed to assay for inducers of FKHR translocation in FKHRGFP fusion protein expressing cells. Monitoring the translocation of the fusion protein from the cytosol to the nucleus requires a high resolution imaging technique....View PDF
Image-based Quantification of Neurite Outgrowth using the Opera
The regeneration of neurons represents a promising strategy for drugs targeted against neurodegenerative injuries and disorders such as Alzheimer’s and Parkinson’s disease [Geerts et al., 2005]. Therefore, the development of new therapies is focused on identifying molecules that affect the differentiation of neurons and neurite outgrowth. For this purpose automated measurement and analysis of neuronal cells is essential for neuroscience research and drug discovery...View PDF
Image-based Quantification of Endothelin A Receptor (ETAR) Translocation using the Opera
Endothelins (ETs) are a class of peptide hormones with strong vasoactive properties [Bagnato & Natali, 2004]. ETs function by interacting with their G protein-coupled receptors
(GPCRs). There are two known ET subtypes, ETA and ETB [Bremnes et al., 2000] The ETA receptor (ETAR) is localized on the plasma membrane until stimulated by endothelins such as endothelin-1 (ET-1), towards which ETAR shows the highest binding affinity...View PDF
Image-based Quantification of Calcium Flux using the Opera
G protein-coupled receptors (GPCRs) comprise one of the largest protein families encoded by the human genome and are a target for approximately 40% of all approved drugs [Eglen et al., 2007]. GPCRs sense molecules outside the cell and activate intracellular signal transduction pathways which ultimately lead to different cellular responses, most of which are linked to calcium signaling...View PDF
Compound Cytotoxicity
Image-based Quantification of Cyclin B1 during Cell Cycle
See the full application note.
Cyclin B1 during Cell Cycle
Image-based Quantification of Cyclin B1 during Cell Cycle
See the full application note.
Apoptosis by Caspase-3
Image-based Quantification of Apoptosis by Caspase-3 Activation
See the full application note.
Nuclear Fragmentation
Image-based Quantification of Apoptosis-Driven Nuclear Fragmentation
See the full application note.
Btk-PH Domain Redistribution®
The Btk-PH Domain Redistribution® Assay
See the full application note.
Beta 2-Adrenoreceptor (ß2AR)
A functional assay for the Beta 2-Adrenoreceptor (ß2AR)
See the full application note.